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MR-compatible optical microscope for in-situ dual-mode MR-optical microscopy.

Matthias C Wapler1, Frederik Testud2, Patrick Hucker2

  • 1Department of Microsystemes Engineering (IMTEK), Laborarory for Microactuators, University of Freiburg, Freiburg, Germany.

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We developed a novel dual-mode microscope combining optical and magnetic resonance microscopy for small animals. This advanced imaging platform offers high resolution and minimal magnetic distortion, enabling clearer biological insights.

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Area of Science:

  • Biomedical Imaging
  • Microscopy
  • Magnetic Resonance Imaging

Background:

  • Simultaneous multimodal imaging offers enhanced biological insights.
  • Integrating optical microscopy with magnetic resonance microscopy presents technical challenges.

Purpose of the Study:

  • To develop and characterize a dual-mode imaging platform combining optical microscopy and magnetic resonance microscopy.
  • To ensure MR-compatibility and minimize image noise for high-quality concurrent imaging.

Main Methods:

  • Designed a microscope for a 9.4T small animal scanner with adaptable RF coils.
  • Implemented electromagnetic shielding using a waveguide for camera protection.
  • Utilized an adaptive lens, modular objectives, and RGBW illumination for optical imaging.
  • Achieved plano-apochromatic optical aberration correction with resolutions from 0.6μm to 2.3μm.

Main Results:

  • Minimized magnetic field inhomogeneity to 19 ppb RMS near the sample.
  • Kept noise increase in MR and optical images below practical relevance.
  • Demonstrated concurrent imaging capabilities with high resolution and minimal distortion.

Conclusions:

  • The developed dual-mode microscope is a viable platform for advanced small animal research.
  • The design strategies overcome key challenges in integrating optical and MR microscopy.
  • This technology enables novel investigations requiring simultaneous high-resolution optical and MR data.