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Impacts of Specific Cryoprotectants on Sperm Freezing and Relationships Between Cryodamage and Oxidation Stress
O Varıslı1, S Erat2, F Bozkaya3
1Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Kırıkkale University, Kırıkkale, Turkey. omervarisli@kku.edu.tr.
Background:
The role of oxidative stress during cryoprotectant treatment has received little attention. OBJECTİVE: To assess the effects of different cryoprotectants and discover relationships between cryodamage and oxidative stress parameters on Awassi ram sperm.
Materials And Methods:
The sperm samples diluted with Salamon's tris-citrate (TRIS) containing 20% centrifuged egg yolk and 0.5, 1.0 or 1.5 M Glycerol (Gly), methanol (M), 2-methoxyethanol (2-ME), dimethylacetamide (DMA) and 1.2 propanediol (PR). After 2 h of equilibration at +4 ºC, the sperm samples were frozen in liquid nitrogen vapour and stored.
Results:
The best post-thaw motility (43.3%, 41.7%) of sperm was achieved when protected with 0.5 and 1.0 M glycerol. Arylesterase and ceruloplasmin parameters were significantly different after equilibration, whereas sulfhydryl groups were significantly different after freezing in their respective groups (P < 0.05).
Conclusion:
The increased use of glycerol caused greater loss of motility. The role of oxidative stress in freezing was also found to be limited.
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