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Updated: Nov 6, 2025
10:36
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
Published on: August 3, 2018
12.3K
Sequencing Ultrarare Targets with Compound Nucleic Acid Cytometry
Chen Sun1, Kai-Chun Chang1, Adam R Abate1,2,3
1Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California 94158, United States.
Analytical Chemistry
|May 10, 2021
Summary
This study introduces a novel cytometry method for highly sensitive, cost-effective targeted sequencing. It achieves million-fold enrichment of long DNA molecules, proving useful for rare target detection in research and clinical settings.
Area of Science:
- Molecular Biology
- Genomics
- Biotechnology
Background:
- Targeted sequencing offers sensitive and cost-effective analysis.
- Current methods face limitations in enrichment power and target capture length.
Purpose of the Study:
- To develop a novel method for enhanced enrichment and long-read capture in targeted sequencing.
- To demonstrate the utility of this method for rare target sequencing applications.
Main Methods:
- Utilized compound nucleic acid cytometry for molecular enrichment.
- Achieved million-fold enrichments for molecules greater than 10 kbp.
- Applied the method to sequence HIV proviruses in infected individuals.
Main Results:
- Demonstrated a novel method for targeted sequencing with high enrichment power.
- Successfully sequenced rare targets, such as HIV proviruses.
- The method requires minimal prior target information.
Conclusions:
- The developed compound nucleic acid cytometry method significantly advances targeted sequencing capabilities.
- This approach is valuable for rare target sequencing in diverse research and clinical applications.
- Potential applications include cancer mutation identification and viral sequencing.
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