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CircATRNL1 protects against osteoarthritis by targeting miR-153-3p and KLF5

Kai-Fu Wang1, Zuo-Wei Shi1, Da-Ming Dong1

  • 1Department of Orthopaedics, the 1st Affiliated Hospital of Harbin Medical University, Harbin 150001, China.

International Immunopharmacology
|May 10, 2021
PubMed
Abstract

Insights

Circular RNA ATRNL1 (circATRNL1) protects against osteoarthritis by inhibiting chondrocyte apoptosis and extracellular matrix degradation via the miR-153-3p/KLF5 pathway. This study highlights circRNAs as potential therapeutic targets for osteoarthritis.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Osteoarthritis (OA) involves chondrocyte injury, with circular RNAs (circRNAs) implicated in its pathogenesis.
  • This study investigates the role of circATRNL1 in osteoarthritis pathology in vitro.

Purpose of the Study:

  • To determine the potential role of circATRNL1 in osteoarthritis (OA) pathology in vitro.
  • To elucidate the molecular mechanisms underlying circATRNL1's function in OA.

Main Methods:

  • Human chondrocytes were treated with IL-1β to simulate OA in vitro.
  • RNA sequencing identified differentially expressed circRNAs, miRNAs, and mRNAs.
  • Quantitative real-time PCR, flow cytometry, and Western blot assays were used for expression analysis and functional assessments.

Main Results:

  • CircATRNL1 expression was reduced in IL-1β-treated chondrocytes.
  • Overexpression of circATRNL1 reduced chondrocyte apoptosis and extracellular matrix (ECM) degradation.
  • CircATRNL1 directly targets miR-153-3p, which in turn regulates KLF5, mediating the observed effects.

Conclusions:

  • CircATRNL1 ameliorates OA development by regulating the miR-153-3p/KLF5 axis.
  • This research enhances understanding of circRNAs as potential therapeutic targets for osteoarthritis treatment.
Keywords:
IL-1βOsteoarthritiscircATRNL1miR-153-3p

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