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miR-133b inhibits cell proliferation, migration, and invasion of lung adenocarcinoma by targeting CDCA8

Chunxiu Hu1, Jianxiang Wu2, Li Wang1

  • 1Department of Cancer Radiotherapy and Chemotherapy, Zhejiang Quhua Hospital, Quzhou, 324004, China.

Pathology, Research and Practice
|May 10, 2021
PubMed
Abstract

Insights

Cell division cycle associated 8 (CDCA8) promotes lung adenocarcinoma (LUAD) growth and spread. MicroRNA-133b (miR-133b) inhibits LUAD by targeting CDCA8, offering a potential therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Lung adenocarcinoma (LUAD) is a prevalent cancer type.
  • Understanding the molecular mechanisms driving LUAD progression is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role of CDCA8 in LUAD cell proliferation, invasion, and migration.
  • To elucidate the regulatory relationship between CDCA8 and miR-133b in LUAD.
  • To explore potential therapeutic targets for LUAD.

Main Methods:

  • Analysis of The Cancer Genome Atlas (TCGA) LUAD dataset for gene expression and survival.
  • Quantitative reverse transcription PCR (qRT-PCR) and Western blot to assess CDCA8 and miR-133b expression.
  • In vitro assays (CCK-8, scratch healing, Transwell) to evaluate cell behavior.
  • Bioinformatic prediction and dual-luciferase reporter assay to confirm miR-133b targeting of CDCA8.

Main Results:

  • CDCA8 was significantly overexpressed in LUAD tissues, while miR-133b was downregulated.
  • miR-133b directly targets and binds to CDCA8.
  • Overexpression of CDCA8 enhanced LUAD cell proliferation, invasion, and migration.
  • miR-133b overexpression reversed the promoting effects of CDCA8.

Conclusions:

  • CDCA8 acts as an oncogene in LUAD, promoting tumor progression.
  • miR-133b functions as a tumor suppressor by inhibiting LUAD cell proliferation, invasion, and migration via targeting CDCA8.
  • The miR-133b/CDCA8 axis represents a potential therapeutic target for LUAD.
Keywords:
CDCA8InvasionMigrationProliferationmiR-133b

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