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Published on: October 11, 2018
tRNA-derived fragments as novel potential biomarkers for relapsed/refractory multiple myeloma
Cong Xu1, Ting Liang2, Fangrong Zhang1
1Department of Hematology, The Third Xiangya Hospital of Central South University, Changsha, 410000, China.
Background:
tRNA-derived fragments have been reported to be key regulatory factors in human tumors. However, their roles in the progression of multiple myeloma remain unknown.
Results:
This study employed RNA-sequencing to explore the expression profiles of tRFs/tiRNAs in new diagnosed MM and relapsed/refractory MM samples. The expression of selected tRFs/tiRNAs were further validated in clinical specimens and myeloma cell lines by qPCR. Bioinformatic analysis was performed to predict their roles in multiple myeloma progression.We identified 10 upregulated tRFs/tiRNAs and 16 downregulated tRFs/tiRNAs. GO enrichment and KEGG pathway analysis were performed to analyse the functions of 1 significantly up-regulated and 1 significantly down-regulated tRNA-derived fragments. tRFs/tiRNAs may be involved in MM progression and drug-resistance.
Conclusion:
tRFs/tiRNAs were dysregulated and could be potential biomarkers for relapsed/refractory MM.
Insights
Dysregulated tRNA-derived fragments (tRFs/tiRNAs) were identified in multiple myeloma (MM). These fragments may play roles in MM progression and drug resistance, suggesting potential as biomarkers for relapsed/refractory MM.
Area of Science:
- Molecular Biology
- Oncology
- Genomics
Background:
- tRNA-derived fragments (tRFs/tiRNAs) are recognized as critical regulatory factors in various human cancers.
- The specific involvement and function of tRFs/tiRNAs in the pathogenesis and progression of multiple myeloma (MM) have not been elucidated.
- Understanding these roles is crucial for developing novel therapeutic strategies and diagnostic tools for MM.
Purpose of the Study:
- To investigate the expression profiles of tRFs/tiRNAs in newly diagnosed and relapsed/refractory multiple myeloma (MM) patient samples.
- To explore the potential roles of dysregulated tRFs/tiRNAs in MM progression and drug resistance.
- To assess the utility of tRFs/tiRNAs as potential biomarkers for relapsed/refractory MM.
Main Methods:
- RNA sequencing was utilized to comprehensively analyze tRF/tiRNA expression patterns in MM patient samples.
- Quantitative real-time PCR (qPCR) was employed for validation of selected tRF/tiRNA expression in clinical specimens and MM cell lines.
- Bioinformatic analyses, including Gene Ontology (GO) enrichment and KEGG pathway analysis, were performed to predict the functional implications of identified tRFs/tiRNAs.
Main Results:
- A significant number of tRFs/tiRNAs exhibited differential expression in MM, with 10 upregulated and 16 downregulated.
- Functional enrichment analyses suggested that these tRFs/tiRNAs are implicated in key pathways related to MM progression and acquired drug resistance.
- Specific tRFs/tiRNAs were identified as significantly altered in relapsed/refractory MM compared to newly diagnosed cases.
Conclusions:
- tRNA-derived fragments (tRFs/tiRNAs) are demonstrably dysregulated in multiple myeloma.
- The identified tRFs/tiRNAs show potential as diagnostic and prognostic biomarkers, particularly for relapsed/refractory MM.
- Further research into the functional mechanisms of these tRFs/tiRNAs could unveil new therapeutic targets for MM treatment.
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