Related Experiment Video
Updated: Nov 6, 2025
07:18
High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
Published on: January 22, 2019
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Shocking HIV-1 with immunomodulatory latency reversing agents
Anna Kula-Pacurar1, Anthony Rodari2, Gilles Darcis3
1Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
Seminars in Immunology
|May 11, 2021
Summary
The "shock-and-kill" strategy aims to cure HIV-1 by reactivating latent virus with latency reversing agents (LRAs) and eliminating infected cells. Understanding how immunomodulatory LRAs work is key to developing better HIV cure strategies.
Area of Science:
- Immunology
- Virology
- Molecular Biology
Background:
- The
- shock-and-kill
- strategy is a leading approach for an HIV-1 cure, targeting latent viral reservoirs.
- This strategy involves reactivating dormant HIV-1 (shock) and eliminating infected cells (kill).
Purpose of the Study:
- To investigate the molecular mechanisms of immunomodulatory latency reversing agents (LRAs) in reversing HIV-1 latency.
- To address the incomplete understanding of how these LRAs function in HIV-1 cure strategies.
Main Methods:
- Review of studies on immunomodulatory LRAs, including immune checkpoint blockers, toll-like receptor agonists, cytokines, and CD8+ T cell depleting antibodies.
Main Results:
- Immunomodulatory LRAs show potential in reactivating latent HIV-1 through various cellular pathways.
- The precise molecular mechanisms underlying LRA-mediated latency reversal are not fully understood.
Conclusions:
- Deciphering the mechanisms of immunomodulatory LRAs is crucial for developing more effective and safer HIV-1 cure strategies.
- The heterogeneity of HIV-1 latency presents a significant challenge in designing successful cure approaches.
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