Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

869
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
869
Antigen Processing Pathways01:31

Antigen Processing Pathways

1.6K
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CD99-mediated immunological synapse formation potentiates CAR-T cell function.

Nature communications·2025
Same author

Vaccine Strategy That Enhances the Protective Efficacy of Systemic Immunization by Establishing Lung-Resident Memory CD8 T Cells Against Influenza Infection.

Immune network·2023
Same author

In vivo delivery of CRISPR-Cas9 using lipid nanoparticles enables antithrombin gene editing for sustainable hemophilia A and B therapy.

Science advances·2022
Same author

A "Prime and Deploy" Strategy for Universal Influenza Vaccine Targeting Nucleoprotein Induces Lung-Resident Memory CD8 T cells.

Immune network·2021
Same author

Adenovirus Vectors: Excellent Tools for Vaccine Development.

Immune network·2021
Same author

Superior immune responses induced by intranasal immunization with recombinant adenovirus-based vaccine expressing full-length Spike protein of Middle East respiratory syndrome coronavirus.

PloS one·2019
Same journal

Oxidative stress drives liver failure during in vivo partial reprogramming.

Molecules and cells·2026
Same journal

scpp5 regulates tooth development and injury-induced repair in zebrafish through mineralization and Wnt/β-catenin signaling.

Molecules and cells·2026
Same journal

Corrigendum to "Molecular role of developmentally regulated GTP-binding protein 1 in coordinating osteoclast and osteoblast differentiation during bone remodeling" [Mol. Cells 49 (2026) 100342].

Molecules and cells·2026
Same journal

Multiple ShKT domain-containing MUL-1 proteins act as redox-responsive modulators of oxidative stress signaling in C. elegans.

Molecules and cells·2026
Same journal

Small molecule inhibition of voltage dependent anion channel 1 reroutes mitochondrial metabolite flux.

Molecules and cells·2026
Same journal

Adherent-to-suspension transition modulates circulating tumor cell dynamics and metastatic potential in melanoma.

Molecules and cells·2026
See all related articles

Related Experiment Video

Updated: Nov 6, 2025

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

10.0K

MHC multimer: A Molecular Toolbox for Immunologists.

Jun Chang1

  • 1Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, Korea.

Molecules and Cells
|May 11, 2021
PubMed
Summary
This summary is machine-generated.

Major histocompatibility complex (MHC) multimer technology revolutionizes antigen-specific T cell analysis, enhancing quantification and applications beyond CD8 T cells. This evolving technique offers broad utility in immunology research and diagnostics.

Keywords:
MHC multimerantigen-specific T cells

More Related Videos

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
11:17

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Published on: March 10, 2021

6.6K
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

13.7K

Related Experiment Videos

Last Updated: Nov 6, 2025

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

10.0K
Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin
11:17

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Published on: March 10, 2021

6.6K
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

13.7K

Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Major histocompatibility complex (MHC) multimer technology has become pivotal in immunology.
  • It significantly enhances the quantification and analysis of antigen-specific T cells, particularly CD8 T cells.
  • Its application is expanding to diverse T cell populations, including CD4 T cells, natural killer T cells, and mucosal-associated invariant T cells.

Purpose of the Study:

  • To provide an updated overview of MHC multimer technology.
  • To broaden the understanding of its expanding applications in T cell research.
  • To encourage the wider adoption of this advanced analytical tool.

Main Methods:

  • MHC multimer technology utilizes multivalent interactions to enhance the low affinity of T-cell receptor-MHC/peptide complexes.
  • It enables the quantification and analysis of antigen-specific T cells.
  • Advanced applications include cell sorting, depletion, stimulation, and single-cell classification via DNA barcoding.

Main Results:

  • MHC multimer technology has dramatically improved the measurement and analysis of antigen-specific T cells.
  • The technology's scope has broadened to encompass various T cell types and functional analyses.
  • Its versatility supports diverse research needs, from basic immunology to potential clinical applications.

Conclusions:

  • MHC multimer technology represents a significant advancement in T cell immunology.
  • Its expanding applications and evolving nature underscore its importance in the field.
  • This technology is crucial for detailed analysis and manipulation of T cell populations.