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Updated: Nov 6, 2025

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research
Leonardo Perez de Souza1, Saleh Alseekh2,3, Federico Scossa2,4
1Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany. lperez@mpimp-golm.mpg.de.
Abstract:
Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) variants currently represent the best tools to tackle the challenges of complexity and lack of comprehensive coverage of the metabolome. UHPLC offers flexible and efficient separation coupled with high-sensitivity detection via HRMS, allowing for the detection and identification of a broad range of metabolites. Here we discuss current common strategies for UHPLC-HRMS-based metabolomics, with a focus on expanding metabolome coverage.
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