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EGR1/2 Inhibits Papillary Thyroid Carcinoma Cell Growth by Suppressing the Expression of PTEN and BAX

Hao Guo1, Linlei Zhang2

  • 1Department of General Surgery, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.

Biochemical Genetics
|May 11, 2021
PubMed

Insights

Early Growth Response (EGR) 1 and 2 proteins are downregulated in papillary thyroid carcinoma (PTC), correlating with poor prognosis. Upregulating EGR1/2 inhibits PTC cell proliferation by modulating PTEN and BAX expression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Early Growth Response (EGR) proteins are implicated in cell growth and apoptosis across various cancers.
  • The specific role of EGR1 and EGR2 in papillary thyroid carcinoma (PTC) remains unclear.
  • Understanding EGR1/2 function in PTC is crucial for potential therapeutic strategies.

Purpose of the Study:

  • To investigate the expression patterns and prognostic significance of EGR1/2 in papillary thyroid carcinoma.
  • To determine the effect of EGR1/2 on PTC cell proliferation.
  • To elucidate the molecular mechanisms by which EGR1/2 influences PTC progression, including target gene regulation.

Main Methods:

  • Analysis of EGR1/2 expression and clinical prognosis using The Cancer Genome Atlas (TCGA) database.
  • Cell viability assays (MTT) to assess the impact of EGR1/2 on PTC cell proliferation.
  • Luciferase reporter assays to confirm direct binding of EGR1/2 to target gene promoter regions.

Main Results:

  • EGR1/2 expression was significantly downregulated in PTC tumor tissues compared to adjacent tissues.
  • Lower EGR1/2 expression correlated with a poorer clinical prognosis in PTC patients.
  • Overexpression of EGR1/2 inhibited proliferation in IHH-4 and BCPAP PTC cell lines, while knockdown enhanced it.
  • EGR1 or EGR2 overexpression led to increased expression of phosphatase and tension homolog (PTEN) or Bcl-2-associated X (BAX).
  • Direct binding of EGR1 or EGR2 to the promoter regions of PTEN or BAX was confirmed.

Conclusions:

  • EGR1/2 are significantly downregulated in papillary thyroid carcinoma and are associated with unfavorable prognosis.
  • Altered EGR1/2 expression impacts PTC cell proliferation.
  • EGR1/2 regulate PTC cell proliferation through modulation of PTEN and BAX expression.
Keywords:
Bcl-2-associated X (BAX)Early growth response (EGR) proteinsPTCPhosphatase and tension homolog (PTEN)

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