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Reporter Genes02:11

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
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Perspectives on Neuroscience
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TO THE EDITOR

Vishal Kumar1, Neradi Deepak2, Sarvdeep Singh Dhatt3

  • 1Associate Professor, Department of Orthopaedics, PGIMER, Chandigarh, India.

Spine
|May 11, 2021
PubMed
Summary

No abstract available in PubMed .

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