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Updated: Nov 6, 2025

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
Published on: February 12, 2019
AlphaFold2 predicts the inward-facing conformation of the multidrug transporter LmrP
Diego Del Alamo1, Cédric Govaerts2, Hassane S Mchaourab1
1Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Abstract:
As part of the CASP competition, the protein structure prediction algorithm AlphaFold2 generated multiple models of the proton/drug antiporter LmrP. Previous distance restraints from double electron-electron resonance spectroscopy, a technique which reports distance distributions between spin labels attached to proteins, suggest that one of the lower-ranked models may have captured a conformation that has so far eluded experimental structure determination.
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