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Effect of Cryopreservation on Semen Quality Parameters in Relation to Lipid Peroxidation and Antioxidant Profile in
A Bisla1, R Rautela2, V Yadav3
1Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Uttar Pradesh, India. amarjeetbislav@gmail.com.
Background:
Lipid peroxidation (LPO) due to oxidative stress leads to structural and functional changes in spermatozoa.
Objective:
To evaluate any association of various seminal characteristics at the pre- and post-cryopreservation stages with LPO and total antioxidant capacity (TAC) in Murrah buffalo semen samples.
Materials And Methods:
Sixty-five ejaculates from seven bulls were processed for cryopreservation in liquid nitrogen.
Results:
Only 31 (47.7%) samples were found satisfactory for inclusion in the further artificial insemination. A strong negative correlation was observed between LPO and individual progressive motility, TAC, viability, plasma membrane integrity as well as acrosome integrity of fresh spermatozoa. At the post-thaw stage, post-thaw motility, viability, plasma membrane integrity and acrosome integrity had strong positive correlation with TAC.
Conclusion:
The effort to minimize LPO and enhance TAC shall play a pivotal role in improving buffalo semen quality upon cryopreservation.
