Ti-9Mn β-type alloy exhibits better osteogenicity than Ti-15Mn alloy in vitro

Area of Science:

• Biomaterials Science
• Orthopedic Implants
• Cell Biology

Background:

• Biomedical implants require materials that promote bone growth and integration.
• Titanium alloys are widely used, but their surface properties influence biological responses.
• Understanding the osteogenic potential of different Ti-Mn compositions is crucial for implant development.

Purpose of the Study:

• To compare the osteogenic behavior of Ti-9Mn and Ti-15Mn alloys in vitro.
• To evaluate the influence of alloy composition on osteoblast cell responses.
• To identify a preferred Ti-Mn alloy for biomedical implant applications.

Main Methods:

• Preparation of Ti-9Mn and Ti-15Mn alloys using arc melting.
• Surface characterization including contact angle and surface roughness (Ra).
• In vitro assessment of MG63 osteoblast-like cell behavior: viability, morphology, alkaline phosphatase (ALP) activity, and gene expression (RUNX2, COL1, OC).

Main Results:

• Ti-9Mn exhibited lower contact angle and higher Ra, indicating better osteoconductive potential.
• Significantly increased ALP activity, cell viability, and filopodia/lamellipodia formation on Ti-9Mn compared to Ti-15Mn.
• Elevated expression of osteogenic markers (RUNX2, COL1, OC) on Ti-9Mn.

Conclusions:

• Ti-9Mn alloy demonstrates enhanced osteogenic properties compared to Ti-15Mn.
• Ti-9Mn promotes faster osteoblast differentiation and proliferation.
• Ti-9Mn is a promising candidate material for biomedical implants over Ti-15Mn.