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Updated: Nov 6, 2025

Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
Chromatographic characterization of a silica hydride-based amide stationary phase
Joseph J Pesek1, Maria T Matyska1, Emma Tardiff1
1Department of Chemistry, San Jose State University, San Jose, CA, USA.
Abstract:
An amide phase based on a porous silica hydride support material is tested for retention characteristics in both the reversed-phase and aqueous normal-phase modes. A series of retention maps (capacity factor vs. mobile phase composition) were obtained using reference standards of varying analyte sizes, functionalities, and polarities. An assessment of the specific column selectivity is made and classes of compounds are identified that show high potential for effective retention, resolution, and efficiency when using amide functionalized silica hydride columns for reversed-phase and aqueous-normal phase separation. Several practical applications are presented that illustrate the capabilities of this particular column format.
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