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Updated: Nov 6, 2025
Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Published on: April 21, 2019
A rapid method for protein staining in polyacrylamide gels using water saturated with chloroform
Paweł Piotr Pieta1, Ewa Burchacka2, Aleksandra Śliwa3
1Department of Bionic and Experimental Medical Biology, Poznan University of Medical Sciences, Poznań, Poland.
Abstract:
Polyacrylamide gel electrophoresis, followed by an appropriate staining, is a popular and useful analytical procedure for protein identification and characterization. The aim of this study was to develop a method for protein visualization in polyacrylamide gels that would be alternative to Coomassie blue or silver staining. The proposed method is simple, fast and inexpensive. The optimized protocol for protein staining and visualization takes as little as 6 minutes and utilizes deionized water and chloroform. Fluorescence of proteins is induced by UV light and can be detected with a standard transilluminator.
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