Related Experiment Video
Updated: Aug 12, 2026
Induction and Assessment of Class Switch Recombination in Purified Murine B Cells
Published on: August 13, 2010
Aberrant immunoglobulin gene rearrangement in scid mouse bone marrow cells
K Okazaki1, S Nishikawa, H Sakano
1Department of Microbiology and Immunology, University of California, Berkeley 94720.
Abstract:
We have analyzed Ig gene rearrangement in the immunodeficient mutant mouse, CB-17 scid. Bone marrow stem cells from scid mice were cultured in the in vitro culture system of Whitlock and Witte. Ig gene rearrangement in the scid cells was studied by DNA cloning. Seven DNA clones of Ig H chain JH and DH regions were analyzed by DNA sequencing, and all the clones contained a failure in D-J joining. In the rearranged structure, both DH and JH coding sequences are either partly or completely deleted. Molecular mechanisms causing the aberrant DNA rearrangement are discussed.
