Innovative extraction materials for fiber-in-tube solid phase microextraction: A review

Fiber-in-tube solid-phase microextraction (fiber-in-tube SPME) with short capillary longitudinally packed with fine fibers as extraction device allows direct coupling to high performance liquid chromatography (HPLC) systems to determine weakly volatile or thermally labile compounds. This technique associates the advantages of miniaturized and analytical on-line systems. Major achievements include the use of different capillaries (fused-silica, copper, stainless steel, polyetheretherketone (PEEK), or poly(tetrafluoroethylene) (PTFE)) that are packed with neat fibers (Zylon®, silk, or Kevlar 29®) or fibers (stainless steel, basalt, or carbon) functionalized with selective coatings (aerogels, ionic liquids (ILs), polymeric ionic liquids (PILs), molecularly imprinted polymers (MIPs), layered double hydroxides (LDHs), or conducting polymer). This review outlines the fundamental theory and the innovative extraction materials for fiber-in-tube SPME-HPLC systems and highlights their main applications in environmental and bioanalyses.