Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

4.3K
The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.3K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.4K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.4K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

7.2K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.2K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

3.0K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.0K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

8.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
8.2K
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

7.0K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thermal shift engagement assay for Class IIA histone deacetylase inhibitor screening.

Results in chemistry·2025
Same author

Cell-surface RNA forms ternary complex with RNA-binding proteins and heparan sulfate to recruit immune receptors.

Molecular cell·2025
Same author

HDAC11 Deacetylates BRAF to Regulate Kinase Activity and Cell Proliferation.

ACS chemical biology·2025
Same author

Mechanistic Insights into Nano-Maillard Reaction Products Regulating the Quality of Dried Abalones.

Foods (Basel, Switzerland)·2025
Same author

Irrigation of <i>Suaeda salsa</i> with Saline Wastewater and Microalgae: Improving Saline-Alkali Soil and Revealing the Composition and Function of Rhizosphere Bacteria.

Microorganisms·2025
Same author

Proteomics-Based Trapping to Study Substrates of Histone Deacetylase 6 Catalytic Domain 1.

Biochemistry·2025

Related Experiment Video

Updated: Nov 6, 2025

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.1K

EGFR phosphorylates HDAC1 to regulate its expression and anti-apoptotic function.

Sonali Bahl1,2, Hongbo Ling1,2, Nuwan P N Acharige3

  • 1Department of Biochemistry & Molecular Medicine, The George Washington University School of Medicine & Health Sciences, Washington, DC, USA.

Cell Death & Disease
|May 12, 2021
PubMed
Summary

Epidermal Growth Factor Receptor (EGFR) activity promotes tyrosine phosphorylation of Histone Deacetylase 1 (HDAC1), enhancing its stability and anti-apoptotic function. This EGFR-mediated phosphorylation of HDAC1 may offer a novel cancer treatment target.

More Related Videos

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

8.8K
Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.7K

Related Experiment Videos

Last Updated: Nov 6, 2025

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.1K
Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

8.8K
Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.7K

Area of Science:

  • Molecular Biology
  • Cellular Signaling
  • Cancer Research

Background:

  • Histone Deacetylase 1 (HDAC1) is a key enzyme regulating gene transcription and cellular processes like apoptosis.
  • HDAC1 activity is modulated by post-translational modifications, including serine phosphorylation by protein kinase CK2.
  • Dysregulation of HDAC1 and Epidermal Growth Factor Receptor (EGFR) activity is linked to tumor progression and cancer survival.

Purpose of the Study:

  • To investigate alternative signaling pathways regulating HDAC1 activity.
  • To explore the role of EGFR in modulating HDAC1 function and stability.
  • To identify potential therapeutic targets for cancer treatment based on HDAC1 regulation.

Main Methods:

  • Investigated the effect of EGFR activity on HDAC1 phosphorylation.
  • Identified specific tyrosine phosphorylation sites on HDAC1 mediated by EGFR.
  • Assessed the impact of EGFR-induced phosphorylation on HDAC1 protein stability and anti-apoptotic function.

Main Results:

  • EGFR activity promotes tyrosine phosphorylation of HDAC1, which is crucial for its protein stability.
  • Tyrosine 72 (Tyr72) on HDAC1 is a key EGFR phosphorylation site mediating its anti-apoptotic function.
  • EGFR-dependent HDAC1 tyrosine phosphorylation influences HDAC1's role in cell survival pathways.

Conclusions:

  • EGFR signaling provides an alternative pathway to regulate HDAC1 activity through tyrosine phosphorylation.
  • HDAC1 Tyr72 phosphorylation by EGFR is essential for its anti-apoptotic effects and protein stability.
  • Targeting HDAC1 tyrosine phosphorylation represents a potential strategy for cancer therapeutics by modulating HDAC1 levels and function.