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Related Concept Videos

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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
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Related Experiment Video

Updated: Nov 6, 2025

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
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Dental stem cell banking: Techniques and protocols.

Sanaz Khaseb1, Mahdi Orooji2, Majid Ghasemian Pour3

  • 1Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University (TMU), Tehran, Iran.

Cell Biology International
|May 12, 2021
PubMed
Summary
This summary is machine-generated.

Dental tissue-derived stem cells (DSCs) offer a promising source for patient-specific therapies with low immune rejection risk. This review details essential protocols for DSC isolation and banking to ensure cell viability and quality for clinical use.

Keywords:
cryopreservationdental stem cell bankingdental tissue-derived stem cellguideline

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Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Biotechnology

Background:

  • Dental tissue-derived stem cells (DSCs) are accessible adult stem cells (ASCs) with significant clinical potential.
  • Autologous transplantation of DSCs minimizes immune rejection risks.
  • Long-term stem cell culture faces challenges like senescence and genetic instability, necessitating cell banking.

Purpose of the Study:

  • To provide comprehensive, step-by-step guidance on DSC isolation and banking.
  • To detail protocols for cryopreserved DSCs, from donor selection to quality control.
  • To present practical principles aligned with established guidelines for DSC banking.

Main Methods:

  • Review of existing literature and established guidelines for stem cell banking.
  • Detailed description of DSC isolation techniques.
  • Elaboration on cryopreservation, characterization, and quality control methods for DSCs.

Main Results:

  • Established protocols for DSC isolation from dental tissues.
  • Standardized methods for cryopreservation and long-term storage of DSCs.
  • Quality control measures to ensure the viability and functionality of banked DSCs.

Conclusions:

  • Standardized DSC banking protocols are crucial for successful clinical applications.
  • This review offers practical guidance for establishing and maintaining DSC banks.
  • Adherence to guidelines ensures the quality and safety of cryopreserved DSCs for research and therapy.