Related Experiment Video

Updated: Nov 6, 2025

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts
09:29

Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts

Published on: March 22, 2017

7.6K

Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological

Eva Riechert1, Vivien Kmietczyk1, Frank Stein2

  • 1Department of Cardiology, Angiology and Pneumology, University Hospital Heidelberg, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany; DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, Germany.

Cell Reports
|May 12, 2021
PubMed

Insights

This study identifies 67 RNA-binding proteins (RBPs) in cardiomyocytes, revealing cytoplasmic polyadenylation element-binding protein 4 (Cpeb4) as a key regulator of cardiac growth and remodeling by controlling specific mRNA targets.

Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • Gene Regulation

Background:

  • RNA-binding proteins (RBPs) are crucial for cardiomyocyte function.
  • The specific RBPs involved in cardiac growth responses remain largely uncharacterized.

Purpose of the Study:

  • To comprehensively identify RBPs in healthy and diseased cardiomyocytes.
  • To investigate the role of cytoplasmic polyadenylation element-binding protein 4 (Cpeb4) in cardiac growth and remodeling.

Main Methods:

  • RNA interactome capture was employed to identify RBPs.
  • In vitro and in vivo models were used to study Cpeb4 function.
  • mRNA targets of Cpeb4 were identified and validated.

Main Results:

  • 67 cardiomyocyte-specific RBPs were identified, including contractile proteins.
  • Cpeb4 was identified as a dynamic RBP regulating cardiac growth.
  • Cpeb4 regulates cardiac remodeling by controlling the expression of transcription factors Zeb1 and Zbtb20.

Conclusions:

  • Cpeb4 is a critical regulator of cardiomyocyte function during pathological growth.
  • Cpeb4 controls cardiac remodeling through differential mRNA binding and subsequent gene expression changes.

Keywords:
Cpeb4RBPsZeb1cardiomycoytespathological hypertrophy

More Related Videos

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes09:41

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

Published on: February 24, 2017

8.9K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.3K

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.3K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
14.4K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.8K
Cardiomyopathy IV: Restrictive Cardiomyopathy01:29

Cardiomyopathy IV: Restrictive Cardiomyopathy

Restrictive cardiomyopathy (RCM) is a rare heart muscle disease characterized by impaired ventricular filling due to stiffened ventricular walls, leading to significant diastolic dysfunction.EtiologyRestrictive cardiomyopathy can arise from both inherited and acquired diseases, many of which are systemic. It is categorized into four main types: infiltrative, storage, non-infiltrative, and endomyocardial diseases.Infiltrative diseases, such as amyloidosis, lead to RCM by depositing amyloid...
152
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.1K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
37.1K