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Non-immune immunoglobulin binding by "Haemophilus somnus"
P R Widders1, J W Smith, M Yarnall
1Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
Journal of Medical Microbiology
|August 1, 1988
Summary
Haemophilus somnus non-immensely binds bovine immunoglobulin (Ig) via its Fc region. This interaction is independent of antigen specificity, involving IgG subclasses and IgM.
Area of Science:
- Veterinary Microbiology
- Immunology
Background:
- Haemophilus somnus is a significant pathogen in cattle.
- Understanding bacterial-host interactions is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the mechanism of non-immune immunoglobulin binding by Haemophilus somnus.
Main Methods:
- In-vitro culture of H. somnus with bovine blood or serum.
- Utilizing immunoglobulin (Ig) fragments (Fab and Fc) to pinpoint binding sites.
- Testing antigen specificity using a hapten-specific IgG preparation.
Main Results:
- H. somnus exhibited non-immune binding of bovine immunoglobulin.
- Binding occurred in the Fc region of IgG, independent of the antigen-combining site.
- Both IgG subclasses (IgG1, IgG2) and IgM were bound by the organism.
Conclusions:
- Haemophilus somnus possesses a mechanism for non-immune binding of bovine immunoglobulins.
- This binding involves the Fc region and is not antigen-specific.
- The findings suggest potential immune evasion strategies employed by H. somnus.