Related Experiment Video

Updated: Nov 5, 2025

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
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Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

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Comment on "Nuclear receptor PXR targets AKR1B7 to protect mitochondrial metabolism and renal function in AKI"

Zhilin Luan1,2, Wenhua Ming1, Cong Zhang1

  • 1Advanced Institute for Medical Sciences, Dalian Medical University, Dalian 116044, China.

Science Translational Medicine
|May 13, 2021
PubMed

Insights

The pregnane X receptor (PXR) may not protect mice from acute kidney injury caused by ischemia and reperfusion. Further research is needed to understand PXR

Area of Science:

  • Nephrology
  • Molecular Biology
  • Pharmacology

Background:

  • The pregnane X receptor (PXR) is a nuclear receptor involved in xenobiotic metabolism.
  • PXR activation has been suggested to have protective roles in various organ injuries.
  • Its role in acute kidney injury (AKI) remains unclear.

Purpose of the Study:

  • To investigate the role of the nuclear pregnane X receptor in protecting against ischemia/reperfusion-induced acute kidney injury (AKI) in a mouse model.

Main Methods:

  • Utilized a mouse model of renal ischemia/reperfusion injury.
  • Assessed kidney injury markers and PXR expression levels.
  • Employed pharmacological activation and genetic knockout strategies for PXR.

Main Results:

  • PXR activation did not confer protection against ischemia/reperfusion-induced AKI in wild-type mice.
  • Kidney injury markers were not significantly improved in PXR-activated mice compared to controls.
  • PXR knockout mice showed similar susceptibility to AKI as wild-type mice.

Conclusions:

  • The nuclear pregnane X receptor may not play a protective role in mitigating ischemia/reperfusion-induced acute kidney injury in mice.
  • These findings suggest that PXR is not a viable therapeutic target for this specific type of kidney injury.

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