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Updated: Nov 5, 2025

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells
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TRIM14 regulates melanoma malignancy via PTEN/PI3K/AKT and STAT3 pathways

Jiangyan Chen1, Lin Huang2, Jin Quan1

  • 1Department of Oncology, Jiangjin Central Hospital of Chongqing, Chongqing, China.

Aging
|May 13, 2021
PubMed

Insights

This study reveals that TRIM14 (tripartite motif-containing protein 14) is upregulated in melanoma, promoting cancer progression. Inhibiting TRIM14 suppressed melanoma cell growth and invasion, suggesting it as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Melanoma is an aggressive skin cancer with limited effective treatments.
  • The role of TRIM14 (tripartite motif-containing protein 14) in melanoma pathogenesis is currently unknown.
  • TRIM14 is recognized as an oncogene in various human cancers.

Purpose of the Study:

  • To investigate the functional role of TRIM14 in melanoma.
  • To elucidate the underlying molecular mechanisms of TRIM14 in melanoma progression.
  • To evaluate TRIM14 as a potential therapeutic target for melanoma.

Main Methods:

  • Analysis of TRIM14 expression in melanoma cell lines.
  • Knockdown and overexpression of TRIM14 in melanoma cells.
  • Assessment of cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT).
  • Western blot analysis to detect protein levels and pathway activation (PTEN, AKT, STAT3).
  • In vivo xenograft mouse models.

Main Results:

  • TRIM14 expression was significantly upregulated in melanoma cell lines.
  • TRIM14 knockdown inhibited melanoma cell proliferation, migration, invasion, and EMT.
  • TRIM14 overexpression promoted these aggressive cellular phenotypes.
  • TRIM14 knockdown led to increased PTEN levels, inactivating AKT and STAT3 pathways.
  • Inhibition of AKT or STAT3 partially reversed TRIM14-mediated melanoma malignancy.
  • In vivo studies corroborated these findings.

Conclusions:

  • TRIM14 acts as an oncogene in melanoma by regulating PTEN/AKT/STAT3 signaling.
  • TRIM14 promotes melanoma cell proliferation, migration, invasion, and EMT.
  • TRIM14 represents a promising molecular target for novel melanoma therapies.
Keywords:
AKTSTAT3TRIM14melanoma

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