Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Compensation and colonization offset the impacts of biodiversity loss over time in grasslands.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Histological transformation into pulmonary sarcomatoid carcinoma from lung adenosquamous carcinoma after radical resection of EGFR gene Exon-21 L858R mutation: a case report and literature review.

Frontiers in oncology·2026
Same author

Reproductive toxicity effects and mechanisms of perfluorobutane sulfonate on the Clitellum in Eisenia fetida: Insights from histopathology, multi-omics and biomarker analyses.

Ecotoxicology and environmental safety·2026
Same author

Influence of the C/N ratio on lactic acid production from food waste under high substrate concentration.

Journal of environmental management·2026
Same author

Safety and efficacy of decompressive craniectomy versus standard craniotomy for large acute epidural haematoma with tentorial herniation in China (PREDICT-AEDH): a nationwide, multicentre, open-label, parallel-group, randomised controlled trial.

The Lancet. Neurology·2026
Same author

Halide doping and morphology engineering in bismuth-based MOFs for enhanced water oxygen evolution.

iScience·2026

Related Experiment Video

Updated: Nov 5, 2025

Characterization of SiN Integrated Optical Phased Arrays on a Wafer-Scale Test Station
05:57

Characterization of SiN Integrated Optical Phased Arrays on a Wafer-Scale Test Station

Published on: April 1, 2020

8.2K

Analytical phase optical transfer function for Gaussian illumination and the optimized illumination profiles.

Jianhui Huang, Yijun Bao, Thomas K Gaylord

    Journal of the Optical Society of America. A, Optics, Image Science, and Vision
    |May 13, 2021
    PubMed
    Summary

    A new polynomial fitting method accurately and rapidly calculates the phase optical transfer function (POTF) for tomographic deconvolution phase microscopy. This method optimizes illumination profiles for improved imaging performance.

    More Related Videos

    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
    12:14

    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

    Published on: August 12, 2013

    22.1K
    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
    14:09

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

    Published on: April 7, 2014

    15.9K

    Related Experiment Videos

    Last Updated: Nov 5, 2025

    Characterization of SiN Integrated Optical Phased Arrays on a Wafer-Scale Test Station
    05:57

    Characterization of SiN Integrated Optical Phased Arrays on a Wafer-Scale Test Station

    Published on: April 1, 2020

    8.2K
    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
    12:14

    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

    Published on: August 12, 2013

    22.1K
    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
    14:09

    Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

    Published on: April 7, 2014

    15.9K

    Area of Science:

    • Optical microscopy
    • Image processing
    • Biophysics

    Background:

    • Tomographic deconvolution phase microscopy (TDPM) imaging performance relies on the phase optical transfer function (POTF).
    • The POTF is influenced by the illumination profile, necessitating optimization for enhanced imaging.
    • Current methods for POTF calculation, like numerical integration, can be time-consuming.

    Purpose of the Study:

    • To develop a faster and accurate analytical method for calculating the POTF in TDPM.
    • To optimize illumination profiles for uniform POTF.
    • To introduce a criterion for balanced POTF distribution.

    Main Methods:

    • An analytical calculation method based on polynomial fitting was developed to describe the POTF for general nonuniform axially symmetric illumination.
    • The method was applied to Gaussian and related illumination profiles.
    • A balanced distribution criterion and least-squares minimization were used for POTF optimization, employing relaxed optimal search and tree search algorithms.

    Main Results:

    • The analytical method significantly reduces calculation time compared to numerical integration while maintaining accuracy.
    • Optimized general and Gaussian illumination profiles were determined.
    • Numerical simulations validated the performance of the optimized profiles and the balanced distribution criterion.

    Conclusions:

    • The developed analytical method offers an efficient and accurate approach for POTF calculation in TDPM.
    • Optimized illumination profiles enhance imaging uniformity and performance.
    • The balanced distribution criterion is effective for achieving uniform POTF in microscopy applications.