Ratio of venous-to-arterial PCO₂ to arteriovenous oxygen content difference during regional ischemic or hypoxic...

The purpose of the study was to evaluate the behavior of the venous-to-arterial CO₂ tension difference (ΔPCO₂) over the arterial-to-venous oxygen content difference (ΔO₂) ratio (ΔPCO₂/ΔO₂) and the difference between venous-to-arterial CO₂ content calculated with the Douglas' equation (ΔCCO_2D) over ΔO₂ ratio (ΔCCO_2D/ΔO₂) and their abilities to reflect the occurrence of anaerobic metabolism in two experimental models of tissue hypoxia: ischemic hypoxia (IH) and hypoxic hypoxia (HH). We also aimed to assess the influence of metabolic acidosis and Haldane effects on the PCO₂/CO₂ content relationship. In a vascularly isolated, innervated dog hindlimb perfused with a pump-membrane oxygenator system, the oxygen delivery (DO₂) was lowered in a stepwise manner to decrease it beyond critical DO₂ (DO_2crit) by lowering either arterial PO₂ (HH-model) or flow (IH-model). Twelve anesthetized and mechanically ventilated dogs were studied, 6 in each model. Limb DO₂, oxygen consumption ([Formula: see text]), ΔPCO₂/ΔO₂, and ΔCCO_2D/ΔO₂ were obtained every 15 min. Beyond DO_2crit, [Formula: see text] decreased, indicating dysoxia. ΔPCO₂/ΔO₂, and ΔCCO_2D/ΔO₂ increased significantly only after reaching DO_2crit in both models. At DO_2crit, ΔPCO₂/ΔO₂ was significantly higher in the HH-model than in the IH-model (1.82 ± 0.09 vs. 1.39 ± 0.06, p = 0.002). At DO_2crit, ΔCCO_2D/ΔO₂ was not significantly different between the two groups (0.87 ± 0.05 for IH vs. 1.01 ± 0.06 for HH, p = 0.09). Below DO_2crit, we observed a discrepancy between the behavior of the two indices. In both models, ΔPCO₂/ΔO₂ continued to increase significantly (higher in the HH-model), whereas ΔCCO_2D/ΔO₂ tended to decrease to become not significantly different from its baseline in the IH-model. Metabolic acidosis significantly influenced the PCO₂/CO₂ content relationship, but not the Haldane effect. ΔPCO₂/ΔO₂ was able to depict the occurrence of anaerobic metabolism in both tissue hypoxia models. However, at very low DO₂ values, ΔPCO₂/ΔO₂ did not only reflect the ongoing anaerobic metabolism; it was confounded by the effects of metabolic acidosis on the CO₂-hemoglobin dissociation curve, and then it should be interpreted with caution.