Effect of the interference with DRP1 expression on the biological characteristics of glioma stem cells
Linna Zhang1, Huimei Cao1, Hong Tao1
1Department of Physiology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China.
Abstract:
In the present study, a model of glioma stem cells (GSCs) was established and combined with molecular targeting drugs in order to observe its inhibitory effect on the proliferation and biological characteristics of GSCs, with the aim of providing a potential target for the treatment of glioma. On the basis of a relatively classical induction strategy with neuron induction medium, a large number of GSC-like cells in good condition and globular growth were amplified in vitro, which had the potential to differentiate into neurons, oligodendrocytes and astrocytes/glioma cells. It was observed that the interference with dynamin-related protein 1 expression using Mdivi-1, a mitochondrial mitotic inhibitor, at the optimal concentration, decreased the expression level of stem cell-associated genes, inhibited proliferation and promoted apoptosis in GSCs. The present study provided an experimental basis for a novel strategy of cancer treatment with tumor stem cells as the target.
Insights
This study developed a glioma stem cell (GSC) model to test targeted drug therapies. Inhibiting dynamin-related protein 1 effectively reduced GSC proliferation and promoted apoptosis, offering a new cancer treatment strategy.
Area of Science:
- Neuroscience
- Oncology
- Cell Biology
Background:
- Glioblastoma is a deadly brain tumor often driven by glioma stem cells (GSCs).
- Targeting GSCs is a promising strategy for glioblastoma treatment.
- Developing effective in vitro models of GSCs is crucial for drug discovery.
Purpose of the Study:
- To establish a reliable in vitro model of glioma stem cells (GSCs).
- To investigate the inhibitory effects of targeting dynamin-related protein 1 (DRP1) on GSC proliferation and characteristics.
- To evaluate the potential of targeting GSCs for glioblastoma treatment.
Main Methods:
- Established a GSC model using neuron induction medium for in vitro culture.
- Amplified GSC-like cells exhibiting globular growth and differentiation potential.
- Utilized Mdivi-1, a mitochondrial mitotic inhibitor, to interfere with DRP1 expression.
Main Results:
- Mdivi-1 treatment at optimal concentrations inhibited GSC proliferation.
- Interference with DRP1 expression led to decreased stem cell-associated gene expression.
- Mdivi-1 treatment promoted apoptosis in GSCs.
Conclusions:
- The established GSC model is suitable for studying therapeutic interventions.
- Targeting DRP1 with Mdivi-1 demonstrates significant anti-proliferative and pro-apoptotic effects on GSCs.
- This study provides a basis for novel cancer therapies targeting tumor stem cells.
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