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Related Experiment Video

Updated: Nov 5, 2025

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
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Salivette, a relevant saliva sampling device for SARS-CoV-2 detection.

Monique Melo Costa1,2,3, Nicolas Benoit1,2,3,4, Jerome Dormoi1,2,3

  • 1Unité Parasitologie Et Entomologie, Département Microbiologie Et Maladies Infectieuses, Institut De Recherche Biomédicale Des Armées, Marseille, France.

Journal of Oral Microbiology
|May 14, 2021
PubMed
Summary

Saliva samples collected with Salivettes® show higher sensitivity for detecting SARS-CoV-2, including in asymptomatic individuals, compared to traditional nasopharyngeal swabs (NPS). This standardized saliva test offers a more sensitive alternative for COVID-19 diagnosis.

Keywords:
COVID-19 diagnosisSARS-CoV-2Salivacoronavirus

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Area of Science:

  • Virology and Infectious Diseases
  • Diagnostic Testing
  • Public Health

Background:

  • Nasopharyngeal swabs (NPS) are the gold standard for COVID-19 diagnosis via RT-qPCR but have limitations.
  • Saliva offers a less invasive, self-collectible alternative for SARS-CoV-2 detection.
  • Previous discrepancies in saliva test results have made their use controversial.

Purpose of the Study:

  • To evaluate Salivettes®, a standardized saliva collection device, for SARS-CoV-2 detection.
  • To compare the positivity rates of paired NPS and saliva specimens.
  • To assess the diagnostic accuracy of saliva samples against NPS.

Main Methods:

  • 303 individuals tested for SARS-CoV-2 were enrolled.
  • Paired nasopharyngeal swab (NPS) and saliva samples were collected.
  • RT-qPCR was performed on both specimen types; analysis included symptomatic, asymptomatic, and follow-up groups.

Main Results:

  • Saliva samples showed a higher positive rate (17.2%) compared to NPS (11.6%).
  • Saliva demonstrated a sensitivity of 82.9% and specificity of 91.4% against NPS.
  • 17 individuals tested positive only via saliva, particularly in the follow-up group, suggesting increased sensitivity.

Conclusions:

  • Saliva collected with Salivette® is more sensitive for detecting symptomatic and pre-symptomatic SARS-CoV-2 infections.
  • Standardized saliva collection devices can improve the reliability of saliva-based COVID-19 testing.
  • Saliva testing offers a promising, sensitive alternative to NPS for diagnosing COVID-19.