Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

764.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
764.3K
RNA-seq03:21

RNA-seq

10.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recognition of non-standard base pairs by triplex-forming oligonucleotides containing an expanded genetic alphabet.

Nature communications·2026
Same author

Scenario-driven fluorescent probes for formaldehyde: From food safety testing to biomedical imaging.

Talanta·2026
Same author

PRMT5 is a prognostic-related biomarker associated with the tumor immune microenvironment in lung adenocarcinoma.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2026
Same author

An ORGANELLAR EXONUCLEASE is essential for nitric oxide dependent pollen tube growth responses.

Plant physiology and biochemistry : PPB·2026
Same author

Transient Increase in AT<sub>1</sub>R Expression at the Myocardial Infarct Site Is Associated with Early Fibrotic Remodeling in Infarcted Rat Heart.

International journal of molecular sciences·2026
Same author

Abscisic acid binds to an <i>Arabidopsis thaliana</i> phosphodiesterase and tunes its activity.

Frontiers in plant science·2026
Same journal

Evaluation of cold resistance in pear (<i>Pyrus</i> L.) germplasms: integrating physiological and biochemical responses with anatomical traits under low temperature stress.

PeerJ·2026
Same journal

Evaluation of retinal and choroidal microvasculature parameters by OCTA in patients with premature ovarian insufficiency: a prospective case control study.

PeerJ·2026
Same journal

Development and prognostic evaluation of a combined SII-LNR score in resectable gastric and gastroesophageal junction adenocarcinoma treated with perioperative FLOT: a retrospective single-center study.

PeerJ·2026
Same journal

Validity and reliability evaluation of the Chinese version of the attention-deficit/hyperactivity disorder stigma questionnaire.

PeerJ·2026
Same journal

Relationship between mental disorders and non-traumatic cerebral hemorrhage: cross-sectional analysis and mendelian randomization.

PeerJ·2026
Same journal

Association between intestinal functional disorders and anal fistula: evidence from a retrospective case-control study.

PeerJ·2026
See all related articles

Related Experiment Video

Updated: Nov 5, 2025

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

8.9K

A Python script to merge Sanger sequences.

Cen Chen1,2,3, Bingguo Lu4, Xiaofang Huang5

  • 1Clinical Center for Human Genomic Research, Union Hospital, Huazhong University of Science and Technology, Wuhan, China.

Peerj
|May 14, 2021
PubMed
Summary
This summary is machine-generated.

This study presents a Python script for merging overlapping Sanger sequences, simplifying gene cloning. The tool efficiently assembles multiple sequences in one run, offering a free solution for researchers.

Keywords:
MergePython scriptSanger sequencing

More Related Videos

3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

10.8K
Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance
04:58

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Published on: December 13, 2024

3.3K

Related Experiment Videos

Last Updated: Nov 5, 2025

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

8.9K
3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

10.8K
Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance
04:58

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Published on: December 13, 2024

3.3K

Area of Science:

  • Molecular Biology
  • Bioinformatics

Background:

  • Gene cloning workflows often require the assembly of multiple Sanger sequencing reads.
  • Manual merging of Sanger sequences can be time-consuming and prone to errors.

Purpose of the Study:

  • To develop and present a user-friendly Python script for automated assembly of overlapping Sanger sequences.
  • To provide a free and efficient computational tool to aid in gene cloning processes.

Main Methods:

  • A Python script was developed to identify and utilize overlapping regions between tandem Sanger sequences.
  • The script processes multiple input Sanger sequence files to generate a single, merged consensus sequence.

Main Results:

  • The script successfully merges multiple overlapping Sanger sequences into a single contiguous sequence.
  • Demonstrated ability to produce the merged sequence from input data in a single execution.

Conclusions:

  • The developed Python script offers a simple, free, and effective method for merging Sanger sequences.
  • This tool can significantly streamline the gene cloning process by automating sequence assembly.