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Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
A Python script to merge Sanger sequences
Cen Chen1,2,3, Bingguo Lu4, Xiaofang Huang5
1Clinical Center for Human Genomic Research, Union Hospital, Huazhong University of Science and Technology, Wuhan, China.
Abstract:
Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning.
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