Related Experiment Video
Updated: Nov 5, 2025
06:21
Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer
Published on: May 10, 2024
939
Identification and Validation of SNP-Containing Genes With Prognostic Value in Gastric Cancer via Integrated
Hui Li1, Jing Guo2, Guang Cheng2
1Department of Medcine, Qingdao University, Qingdao, China.
Frontiers in Oncology
|May 14, 2021
Summary
This study identified six key genes (TP53, HRAS, BRCA1, PIK3CA, AKT1, and SMARCA4) linked to gastric cancer survival. These findings offer insights into gastric cancer development and prognosis, aiding future research and patient outcomes.
Area of Science:
- Genomics
- Bioinformatics
- Cancer Research
Background:
- Gastric cancer remains a leading cause of cancer mortality worldwide.
- Despite treatment advances, poor survival rates persist due to recurrence and metastasis.
- Identifying molecular pathogenesis and prognostic markers is crucial for improving patient outcomes.
Purpose of the Study:
- To identify and validate single nucleotide polymorphisms (SNPs) in genes with prognostic significance for gastric cancer.
- To explore the molecular mechanisms underlying gastric cancer development and progression.
Main Methods:
- Utilized The Cancer Genome Atlas (TCGA) for SNP data analysis.
- Constructed protein-protein interaction (PPI) networks using STRING and Cytoscape.
- Analyzed gene expression and prognostic value via UALCAN and Kaplan-Meier Plotter.
- Validated hub gene expression in gastric cancer cells using qPCR and Western blotting.
Main Results:
- Identified 945 mutated genes from TCGA data.
- Constructed a PPI network with 360 nodes and 1616 edges.
- Pinpointed six key genes: TP53, HRAS, BRCA1, PIK3CA, AKT1, and SMARCA4, significantly associated with patient survival.
Conclusions:
- TP53, HRAS, BRCA1, PIK3CA, AKT1, and SMARCA4 are identified as potential key genes in gastric cancer development and prognosis.
- This research provides a bioinformatics and theoretical basis for further investigation into gastric cancer's molecular pathogenesis.
- Findings may aid in developing improved prognostic evaluations for gastric cancer patients.
Related Concept Videos
01:11Genome-wide Association Studies-GWAS
14.8K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
GWAS does not require the identification of the target gene involved in...
14.8K
01:05Single Nucleotide Polymorphisms-SNPs
17.2K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
17.2K