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TNPO1-Mediated Nuclear Import of FUBP1 Contributes to Tumor Immune Evasion by Increasing NRP1 Expression in Cervical

BiKang Yang1, Jing Chen1,2, YinCheng Teng1,2

  • 1Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China.

Journal of Immunology Research
|May 14, 2021
PubMed

Insights

Far upstream element binding protein 1 (FUBP1) promotes cervical cancer (CC) progression by regulating oncogene expression and immune checkpoints. Inhibiting FUBP1 may offer a new therapeutic strategy for CC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Far upstream element binding protein 1 (FUBP1) is a DNA-binding protein involved in oncogenesis.
  • The precise mechanisms of FUBP1 in tumor promotion, particularly in cervical cancer, require further investigation.

Purpose of the Study:

  • To elucidate the role of FUBP1 in cervical cancer (CC) progression.
  • To investigate the molecular mechanisms underlying FUBP1's function in CC.
  • To explore FUBP1 as a potential therapeutic target for CC.

Main Methods:

  • Analysis of FUBP1 mRNA and protein expression in cervical cancer tissues.
  • In vitro functional assays (e.g., knockdown experiments) to assess the impact of FUBP1 on CC cell proliferation and migration.
  • Investigation of FUBP1 nuclear localization and its interaction with Transportin-1 (TNPO1).
  • Assessment of FUBP1's regulation of immune checkpoint NRP1 gene expression.

Main Results:

  • FUBP1 expression is significantly upregulated in cervical cancer and correlates with poor prognosis.
  • FUBP1 knockdown inhibits CC cell proliferation and migration, indicating a pro-oncogenic role.
  • FUBP1 nuclear translocation, mediated by TNPO1 interaction, regulates the expression of the immune checkpoint NRP1.
  • FUBP1 demonstrates a pro-oncogenic function in cervical cancer progression.

Conclusions:

  • FUBP1 plays a critical role in cervical cancer progression by regulating oncogene expression and immune checkpoints.
  • FUBP1 nuclear localization, facilitated by TNPO1, is a key mechanism in its oncogenic activity.
  • FUBP1 represents a promising therapeutic target for restricting cervical cancer progression.

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