Related Experiment Video
Updated: Nov 5, 2025
Handling and Treatment of Male European Eels Anguilla anguilla for Hormonal Maturation and Sperm Cryopreservation
Published on: January 28, 2018
Liquid Storage of Geophagus brasiliensis semen in the Presence of Different Extenders
J S Caldas1, C D Corcini2, E F Silva3
1BAAC, Aquatic Environments Continental Biology, Institute of Biological Sciences, Federal University of Rio Grande, Rio Grande, RS, Brazil.
Background:
In order to preserve the genetic diversity of cichlid fish in gene banks, it is necessary to use certain extenders to maintain the integrity of spermatozoa cells during cooling.
Objective:
To evaluate the effects of different extenders on the quality parameters of cooled semen of Geophagus brasiliensis.
Materials And Methods:
Semen samples were collected from seven adult fish and diluted with five extenders: Beltsville Thawing Solution (BTS™), Hanks' Balanced Salt Solution (HBSS), Tris-glucose, Ginsburg's Fish Ringers, and Phosphate buffered Saline. All parameters were evaluated in fresh semen samples and after cooling at 4°C at 0, 24, 48, and 96 h to evaluate cell viability (membrane integrity, DNA, and mitochondrial functionality) and motility rate and weather motility.
Results:
The BTS and Tris-glucose resulted in the best outcomes (P<0.05) in terms of membrane integrity assessments (35.1% and 30.9%, respectively), DNA integrity (71.6%; 75.7%), mitochondrial function (26.9%; 28.0%) and motility rate (8.6%; 8.6%), respectively, for semen cooled to 4°C for 96 h. However, the 48-h period motility after cooling in BTS was superior to all other treatments.
Conclusion:
BTS and Tris-glucose can be considered as the best extenders for the cold storage of Geophagus brasiliensis spermatozoa.
