Related Experiment Video
Updated: Nov 5, 2025

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System
Published on: September 23, 2021
CO2 -responsive CCT protein interacts with 14-3-3 proteins and controls the expression of starch synthesis-related
Hiroshi Fukayama1, Fumihiro Miyagawa1, Naoki Shibatani1
1Laboratory of Tropical Plant Science, Graduate School of Agricultural Science, Kobe University, Kobe, Japan.
Abstract:
CO2 -responsive CCT protein (CRCT) is a positive regulator of starch synthesis-related genes such as ADP-glucose pyrophosphorylase large subunit 1 and starch branching enzyme I particularly in the leaf sheath of rice (Oryza sativa L.). The promoter GUS analysis revealed that CRCT expressed exclusively in the vascular bundle, whereas starch synthesis-related genes were expressed in different sites such as mesophyll cell and starch storage parenchyma cell. However, the chromatin immunoprecipitation (ChIP) using a FLAG-CRCT overexpression line and subsequent qPCR analyses showed that the 5'-flanking regions of these starch synthesis-related genes tended to be enriched by ChIP, suggesting that CRCT can bind to the promoter regions of these genes. The monomer of CRCT is 34.2 kDa; however, CRCT was detected at 270 kDa via gel filtration chromatography, suggesting that CRCT forms a complex in vivo. Immunoprecipitation and subsequent MS analysis pulled down several 14-3-3-like proteins. A yeast two-hybrid analysis and bimolecular fluorescence complementation assays confirmed the interaction between CRCT and 14-3-3-like proteins. Although there is an inconsistency in the place of expression, this study provides important findings regarding the molecular function of CRCT to control the expression of key starch synthesis-related genes.
More Related Videos
Related Concept Videos
Cell Specific Gene Expression
C4 Pathway and CAM
C4 Pathway
The C4 pathway is used by plants such as...
Regulation of Transpiration by Stomata
Protein Transport to the Stroma
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
The Calvin Benson Cycle
Global Regulatory Systems

