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Patterns of brachyury expression in chordomas.

Maroa Dridi1, Jean Boutonnat2, Jean Marc Dumollard1

  • 1Pathology Departments, University Hospital of Saint-Etienne, France.

Annals of Diagnostic Pathology
|May 14, 2021
PubMed
Summary

Brachyury expression in chordomas is influenced by sample age and decalcification, impacting diagnosis. Standardizing immunohistochemical studies, including antibody clone and sample handling, is crucial for consistent results in chordoma research.

Keywords:
Brachyury, ImmunohistochemistryChordomaCloneDecalcificationNotochordTissue Age

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Area of Science:

  • Oncology
  • Pathology
  • Molecular Biology

Background:

  • Chordomas are rare malignant midline tumors linked to notochordal remnants.
  • Brachyury's role in chordoma pathogenesis is established, with its immunohistochemical expression vital for diagnosis.
  • Variability in primary antibodies used in studies hinders comparability of brachyury expression data.

Purpose of the Study:

  • To investigate brachyury expression in chordomas.
  • To correlate brachyury expression with clinicopathological characteristics.
  • To review literature on immunohistochemical brachyury studies for standardization.

Main Methods:

  • Retrospective analysis of 62 chordoma cases diagnosed over 22 years.
  • Used a monoclonal primary antibody (clone A-4) and H-score for brachyury evaluation.
  • Analyzed clinicopathological parameters including age, sex, localization, decalcification, and tissue age; fetal notochords used for comparison.

Main Results:

  • Mean H-score for nuclear brachyury expression was 129.8.
  • Older tissue samples showed reduced brachyury expression; decalcification tended to weaken expression.
  • No correlation found between clinical characteristics and brachyury expression patterns; fetal notochords were negative.

Conclusions:

  • Uniformity in brachyury immunohistochemical studies is necessary for chordoma diagnosis and treatment.
  • Standardization should consider antibody clone, decalcification, and sample age.
  • Brachyury's diagnostic and therapeutic importance necessitates consistent research methodologies.