Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Diet-Microbiome-Immune Interactions at the Gut Mucosa in Food Allergy: Mechanisms, Gaps, and Therapeutic Implications.

Allergy·2026

Same author

Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

Journal of immunological methods·2026

Same author

Algorithms of Patient Selection for Allergen Immunotherapy.

Allergy·2026

Same author

Evaluating Basophil Histamine Content as a Biomarker for Omalizumab Responsiveness in Chronic Spontaneous Urticaria.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2026

Same author

Proteomic analysis of horse hair extracts provides no evidence for the existence of a hypoallergenic Curly Horse breed.

Clinical and translational allergy·2026

Same author

Allergic Rhinitis and Its Impact on Asthma (ARIA)-EAACI Guidelines-2024-2025 Revision: Part II-Guidelines on Oral and Ocular Treatments.

Allergy·2026

Same journal

GWAS of Second-Generation Antihistamine Use: Locus Specificity and Highlighted Signalling Pathways.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2026

Same journal

Basophil Activation Test Provides Additional Diagnostic Value Beyond Ara h 2-Specific IgE Testing in Peanut Allergic Adults.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2026

Same journal

Characterisation of Allergen Profiles in Commercial Egg Products to Inform Oral Immunotherapy Strategies in Egg Allergy.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2026

Same journal

Allergic Diseases and Risk of Incident Autoimmune Diseases: Phenotype-Specific Patterns and Multimorbidity Effects in a Nationwide Cohort.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2026

Same journal

Determinants of Total and Inhaled Allergen-Specific Immunoglobulin E in the Middle-Aged and Elderly Population.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2026

Same journal

Rhinitis-Related Healthcare Utilization in Colombian Adults, 2015-2024.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 5, 2025

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

A novel method for quantifying ingested food allergens in human sera

Per Stahl Skov^1,2, Esben Eller^1,3, Niels Peter Knudsen²

¹Department of Dermatology & Allergy Center, Odense University Hospital, Odense Research Center for Anaphylaxis (ORCA, Odense, Denmark.

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology

|May 15, 2021

Summary

No abstract available in PubMed .

More Related Videos

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

Published on: June 4, 2017

Related Experiment Videos

Last Updated: Nov 5, 2025

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

Published on: June 4, 2017