Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.4K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.4K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.4K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.4K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

24.8K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
24.8K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

4.8K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.8K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.9K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K
Embryonic Stem Cells00:57

Embryonic Stem Cells

4.2K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Continuous modeling of primate embryogenesis from totipotency to early organogenesis.

Cell·2026
Same author

The mechanism of action and therapeutic potential of macrophages in osteoporosis: from polarization balance to targeted regulation.

Frontiers in immunology·2026
Same author

Learning curve of laparoscopic duodenum-preserving total pancreatic head resection: a single-center dual CUSUM and risk-adjusted CUSUM analysis.

Surgical endoscopy·2026
Same author

Analysis and application of microbiota in fermentation pit muds used for Chinese strong-flavor liquor production.

AMB Express·2026
Same author

Comparative Severe Complications After Laparoscopic Pancreatoduodenectomy in PDAC vs Non-PDAC Periampullary Cancer: A Retrospective Cohort Study.

Journal of surgical oncology·2026
Same author

Risk factors of reoperation after laparoscopic pancreaticoduodenectomy: insight from a learning curve analysis.

Updates in surgery·2026

Related Experiment Video

Updated: Nov 5, 2025

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.6K

Mouse totipotent stem cells captured and maintained through spliceosomal repression.

Hui Shen1, Min Yang2, Shiyu Li1

  • 1MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking University, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.

Cell
|May 15, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a method to create totipotent stem cells (TSCs) in vitro. By inhibiting splicing in mouse embryonic stem cells (ESCs), they generated TSCs with full developmental potential, advancing stem cell research.

Keywords:
chimerasembryonicembryonic stem cellextraembryonicpladienolide Bpluripotentspliceosomesplicingtotipotenttranscriptome

More Related Videos

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

12.5K
The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

7.2K

Related Experiment Videos

Last Updated: Nov 5, 2025

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.6K
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

12.5K
The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

7.2K

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Establishing in vitro cultures of totipotent cells comparable to in vivo blastomeres has been a significant challenge.
  • Embryonic stem cells (ESCs) possess pluripotency but lack the full developmental potential of early blastomeres.

Purpose of the Study:

  • To investigate the role of spliceosomal activity in regulating the transition from pluripotency to totipotency.
  • To develop a method for stable in vitro culture of totipotent cells.

Main Methods:

  • Utilized pladienolide B, a splicing inhibitor, to modulate spliceosomal activity in mouse ESCs.
  • Employed mouse chimeric assays and single-cell RNA sequencing (sc-RNA seq) to assess developmental potential and molecular characteristics.
  • Analyzed gene splicing patterns of pluripotent and totipotent genes.

Main Results:

  • Achieved stable in vitro culture of totipotent blastomere-like cells (TBLCs) from mouse ESCs.
  • TBLCs demonstrated robust bidirectional developmental capability, generating both embryonic and extraembryonic lineages.
  • Spliceosomal repression inhibited splicing of pluripotent genes while activating totipotent genes with short introns.

Conclusions:

  • Spliceosomal repression is a key mechanism driving the transition of ESCs to a totipotent state.
  • The developed method enables the capture and maintenance of totipotent stem cells in vitro.
  • This breakthrough offers new possibilities for studying early embryonic development and regenerative medicine.