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Updated: Nov 5, 2025
Author Spotlight: Development of a Smartphone-Enhanced Paper-Based Device for Rapid Dengue NS1 Detection
Published on: January 26, 2024
Clinical evaluation of a developed paper-based Dengue NS1 rapid diagnostic test for febrile illness patients
Muhammad Hatta Prabowo1, Supawat Chatchen2, Patsamon Rijiravanich3
1School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bang Khun Thian, Bangkok 10150, Thailand; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia, Sleman, Yogyakarta, 55584, Indonesia.
Objectives:
This study aimed to evaluate a microfluidic paper-based analytical device (DEN-NS1-PAD) based on a rapid NS1 antigen test for diagnosing dengue at the point of care.
Methods:
219 serum samples from suspected dengue cases were tested with the developed DEN-NS1-PAD and commercial RDT by SD BIOLINE. The results were compared with the nested-PCR results.
Results:
The limit of detection of DEN-NS1-PAD was 0.78 ng mL-1. It showed 88.89% sensitivity, 86.67% specificity, and a substantial agreement correlation (κ = 0.7522) compared with nested-PCR. In contrast, SD BIOLINE for NS1 (SD-NS1) detection showed 87.88% sensitivity, 90.00% specificity, and had a substantial agreement correlation with nested-PCR (κ = 0.7788).
Conclusions:
DEN-NS1-PAD is a valuable tool for diagnosing DENV infections, especially for diagnosed patients with early acute phase samples with high viral load. DEN-NS1-PAD has better sensitivity than SD-NS1 but less specificity.
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