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Updated: Nov 5, 2025

Physical, Chemical and Biological Characterization of Six Biochars Produced for the Remediation of Contaminated Sites
Published on: November 28, 2014
Synergetic effects of biochars and denitrifier on nitrate removal
Yuqi Liu1, Shulei Liu1, Zongcai Yang1
1School of the Environment, State Key Laboratory for Pollution Control and Resource Reuse, Nanjing University Xianlin Campus, Nanjing 210023, China.
Abstract:
Nitrate is one of the most common water contaminants and has caused severe environmental problems. This work aimed to investigate the effects of integration of denitrifier with biochars on nitrate removal and understand the underlying mechanisms. The results showed that physiochemical properties of biochars varied according to different feedstocks, which influenced bacteria attachment and nitrate removal through adsorption. However, bacteria could colonize on biochars no matter biochars surface were favorable for bacteria attachment or not. Immobilization of denitrifier on biochars significantly improved nitrate removal efficiencies and reduced lag time. Underlying mechanisms investigation showed that the integration of denitrifier with biochars had synergetic effects on promoting nitrate removal, which improved not only the expression and activity of nitrate reductase, but also the electron transport system activity.
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