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Association of CHUK gene polymorphism and ischemic stroke in the Han Chinese population

Jingyan Huang1, Qiugui Wei2, Baoyun Liang2

  • 1The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 510120 Guangzhou, Guangdong, China; Guangzhou University of Chinese Medicine, 510405 Guangzhou, Guangdong, China; University at Buffalo, The State University of New York, 14228 Buffalo, NY, USA; Guangxi University of Chinese Medicine, 530299 Nanning, Guangxi, China.

Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia
|May 16, 2021
PubMed

Insights

Component of inhibitor of nuclear factor kappa B kinase complex (CHUK) expression is elevated in ischemic stroke (IS) patients. While CHUK gene variants are not linked to IS risk, they may influence blood pressure and triglyceride levels in IS patients.

Area of Science:

  • Genetics and Molecular Biology
  • Cardiovascular Disease Research
  • Neurology

Background:

  • Component of inhibitor of nuclear factor kappa B kinase complex (CHUK) plays a role in lipid levels and blood pressure.
  • Hypertension and hyperlipidemia are established risk factors for ischemic stroke (IS).
  • The direct association between CHUK and IS risk remained unexplored.

Purpose of the Study:

  • To investigate the relationship between CHUK gene polymorphisms (rs3808916, rs2230804, rs3808917) and the risk of ischemic stroke (IS).
  • To evaluate the association of these CHUK polymorphisms with IS-related risk factors, including blood pressure and lipid levels.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure CHUK mRNA expression in 53 IS patients and 53 healthy controls.
  • Genotyping of CHUK polymorphisms (rs3808916, rs2230804, rs3808917) was performed in 816 IS patients and 816 matched healthy controls using Sequenom MassARRAY iPLEX.
  • Haplotype analysis was conducted to assess the combined effect of CHUK polymorphisms.

Main Results:

  • CHUK mRNA expression was significantly higher in IS patients compared to healthy subjects (P < 0.001).
  • No significant association was found between the studied CHUK polymorphisms (rs3808916, rs2230804, rs3808917) and IS susceptibility.
  • CHUK variant rs2230804 showed a relationship with diastolic blood pressure (P = 0.035), and rs3808917 was associated with triglyceride levels (P = 0.046) in IS patients.

Conclusions:

  • Elevated CHUK expression is implicated in the pathogenesis of ischemic stroke.
  • Specific CHUK variants (rs2230804 and rs3808917) may influence diastolic blood pressure and triglyceride levels in individuals with IS.
  • The investigated CHUK polymorphisms (rs3808916, rs2230804, rs3808917) do not appear to be direct risk factors for developing ischemic stroke.
Abstract

Keywords:
CHUKIS-related risk factorsIschemic strokePolymorphismmRNA expression

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