Genotypic diversity of 17 cacti species and application to biosynthesis of gold nanoparticles

The genotypic diversity of 17 cacti species were examined and grouped in four clusters using seven inter simple sequence repeat (ISSR) markers. Group representatives (five species) were chosen for AuNPs synthesis in the cacti syrups. To synthesize the Gold nanoparticles (AuNPs), reducing and capping potential of five species of cacti rich in the polyphenolics were explored. Based on the synthesized AuNPs traits (concentration, pH, temperature, and synthesis time), Opuntia pycnacantha with the highest absorption peak at 540 nm was chosen for further characterizations. Varieties of diffraction peaks confirmed the successful synthesis of AuNPs. AuNPs functionalization with the phenolic compounds was confirmed by Fourier transform infrared (FTIR) spectroscopy. At the optimum conditions (pH = 5.0 and T = 60 °C), both dynamic light scattering (DLS) and transmission electron microscopy (TEM) revealed more than 87% of AuNPs to be 2.5 nm in size with Zeta potential to be equal to -19.9 mV.