Flavobacterium inviolabile sp. nov. isolated from stream water

Area of Science:

• Microbiology
• Bacteriology
• Environmental Science

Background:

• Microbial diversity in freshwater ecosystems is crucial for ecological balance.
• The genus Flavobacterium comprises diverse species with varying environmental roles.
• Isolation and characterization of novel bacteria contribute to understanding microbial genomics and ecology.

Purpose of the Study:

• To isolate and characterize a novel bacterial strain from Moonsan stream water.
• To determine the taxonomic and phylogenetic placement of the novel isolate.
• To propose a new species within the genus Flavobacterium based on polyphasic evidence.

Main Methods:

• Isolation and cultivation of strain P2-65^T from stream water.
• Physiological and biochemical characterization, including growth conditions and fatty acid analysis.
• 16S rRNA gene sequencing, average nucleotide identity (ANI), and digital DNA-DNA hybridization (dDDH) for phylogenetic analysis.

Main Results:

• Strain P2-65^T is a Gram-stain-negative, aerobic, rod-shaped bacterium.
• Phylogenetic analysis based on 16S rRNA gene sequences showed close relatedness to Flavobacterium cauense and Flavobacterium cheniae.
• ANI and dDDH values were below thresholds for species delineation, supporting novelty. Major fatty acids and polar lipids were identified, and the G+C content was 39.7%.

Conclusions:

• Strain P2-65^T represents a novel species of the genus Flavobacterium.
• The proposed name for this new species is Flavobacterium inviolabile sp. nov.
• The type strain is P2-65^T (KCTC 62055^T = NBRC 112953^T).