Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

58.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
58.3K
Alternative RNA Splicing02:18

Alternative RNA Splicing

23.2K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
23.2K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.3K
4.3K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

6.0K
6.0K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

3.0K
3.0K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel Biobased Sodium Shellac for Wrapping Disperse Multiscale Emulsion Particles.

Journal of agricultural and food chemistry·2016
Same author

Psychometric properties of the International Classification of Functioning, Disability and Health set for spinal cord injury nursing based on Rasch analysis.

Disability and rehabilitation·2016
Same author

Future challenges in understanding ROS in plant responses to abiotic stress.

Science China. Life sciences·2016
Same author

FOXD3 is a tumor suppressor of colon cancer by inhibiting EGFR-Ras-Raf-MEK-ERK signal pathway.

Oncotarget·2016
Same author

Adsorption of perfluorooctane sulfonate on soils: Effects of soil characteristics and phosphate competition.

Chemosphere·2016
Same author

Anti-inflammatory effect of cannabinoid agonist WIN55, 212 on mouse experimental colitis is related to inhibition of p38MAPK.

World journal of gastroenterology·2016

Related Experiment Video

Updated: Nov 5, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.2K

Splicing factor SRSF2-centric gene regulation.

Kun Li1, Ziqiang Wang1,2

  • 1Department of Nuclear Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan 250014, China.

International Journal of Biological Sciences
|May 17, 2021
PubMed
Summary
This summary is machine-generated.

Serine/arginine-rich splicing factor 2 (SRSF2) upregulation by HSV-1 infection epigenetically controls viral gene transcription. Upregulated SRSF2 in T cells also influences immune checkpoints, highlighting its role in disease.

Keywords:
SC35SRSF2gene regulationmRNA stability.splicingtranscription

More Related Videos

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.1K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.0K

Related Experiment Videos

Last Updated: Nov 5, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.2K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.1K
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.0K

Area of Science:

  • Molecular Biology
  • Immunology
  • Virology

Background:

  • Serine/arginine-rich splicing factor 2 (SRSF2) is a crucial splicing factor in mammalian cells.
  • SRSF2 plays significant roles in biological and pathological processes.
  • Understanding SRSF2 regulation and its target gene interactions is vital.

Purpose of the Study:

  • To investigate the regulation of SRSF2 expression.
  • To elucidate the mechanisms by which SRSF2 regulates target genes.
  • To explore SRSF2's role in viral infection and T cell exhaustion.

Main Methods:

  • Analysis of SRSF2 expression changes during herpes simplex virus-1 (HSV-1) infection.
  • Investigation of epigenetic regulation of HSV-1 genes by SRSF2.
  • Study of SRSF2's association with P300/CBP and histone modifications in exhausted T cells.

Main Results:

  • SRSF2 expression is upregulated by HSV-1 infection.
  • Altered SRSF2 epigenetically regulates HSV-1 gene transcription.
  • Upregulated SRSF2 in exhausted T cells increases immune checkpoint molecules via histone modification.

Conclusions:

  • SRSF2 acts as a key regulator in gene expression.
  • SRSF2 is an important sensor and effector in disease progression.
  • SRSF2 influences viral gene transcription and immune responses.