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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Detection of circRNA Biomarker for Acute Myocardial Infarction Based on System Biological Analysis of RNA Expression
1Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.
Insights
Researchers identified a novel circular RNA (circRNA) as a promising biomarker for diagnosing and treating acute myocardial infarction (AMI). This finding could improve early detection and patient outcomes for this serious heart condition.
Area of Science:
- Cardiovascular Biology
- Molecular Diagnostics
- Biomarker Discovery
Background:
- Acute myocardial infarction (AMI) is a leading cause of mortality, necessitating improved diagnostic tools.
- Circular RNAs (circRNAs) are emerging as potential biomarkers for cardiovascular diseases.
- Early diagnosis and targeted therapy are crucial for managing AMI.
Purpose of the Study:
- To identify novel circRNA biomarkers for acute myocardial infarction (AMI).
- To investigate the diagnostic and therapeutic potential of identified circRNAs in AMI.
- To establish a circRNA as a candidate biomarker for AMI.
Main Methods:
- Comparative analysis of circRNA expression in blood samples from AMI patients and controls.
- Construction of a circRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network.
- Validation using fluorescence in situ hybridization, cardiomyocyte models, and gene manipulation techniques.
Main Results:
- Differential expression of specific circRNAs was observed in AMI patients.
- A key circRNA was identified through ceRNA network analysis as significantly associated with AMI.
- Experimental validation confirmed the candidate circRNA's role in AMI pathogenesis and its potential as a diagnostic marker.
Conclusions:
- A specific circRNA has been identified as a potential effective biomarker for AMI diagnosis.
- The findings suggest circRNAs can serve as valuable therapeutic targets for cardiovascular diseases.
- This research contributes to the development of novel strategies for managing acute myocardial infarction.
Abstract:
Acute myocardial infarction (AMI) is myocardial necrosis caused by the persistent interruption of myocardial blood supply, which has high incidence rate and high mortality in middle-aged and elderly people in the worldwide. Biomarkers play an important role in the early diagnosis and treatment of AMI. Recently, more and more researches confirmed that circRNA may be a potential diagnostic biomarker and therapeutic target for cardiovascular diseases. In this paper, a series of biological analyses were performed to find new effective circRNA biomarkers for AMI. Firstly, the expression levels of circRNAs in blood samples of patients with AMI and those with mild coronary stenosis were compared to reveal circRNAs which were involved in AMI. Then, circRNAs which were significant expressed abnormally in the blood samples of patients with AMI were selected from those circRNAs. Next, a ceRNA network was constructed based on interactions of circRNA, miRNA and mRNA through biological analyses to detect crucial circRNA associated with AMI. Finally, one circRNA was selected as candidate biomarker for AMI. To validate effectivity and efficiency of the candidate biomarker, fluorescence in situ hybridization, hypoxia model of human cardiomyocytes, and knockdown and overexpression analyses were performed on candidate circRNA biomarker. In conclusion, experimental results demonstrated that the candidate circRNA was an effective biomarker for diagnosis and therapy of AMI.
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