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Updated: Nov 5, 2025

A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model
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MicroRNA in Human Acute Kidney Injury: A Systematic Review Protocol

Adrianna Douvris1, Dylan Burger1,2, Rosendo A Rodriguez3

  • 1Division of Nephrology, Department of Medicine and Kidney Research Centre, Ottawa Hospital Research Institute, The University of Ottawa and The Ottawa Hospital, ON, Canada.

Canadian Journal of Kidney Health and Disease
|May 17, 2021
PubMed
Abstract

Insights

This systematic review evaluates microRNAs (miRNAs) in human acute kidney injury (AKI). It identifies promising miRNAs and research constraints to guide future studies on AKI prevention and treatment.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nephrology

Background:

  • Acute kidney injury (AKI) is a significant cause of hospitalization with high mortality and limited treatment options.
  • Current diagnostic tools for AKI have limitations in early identification.
  • MicroRNAs (miRNAs) are implicated in AKI pathogenesis, with potential therapeutic roles shown in animal models, but their role in human AKI is less understood.

Purpose of the Study:

  • To systematically review and analyze the role of microRNAs (miRNAs) in human subjects diagnosed with acute kidney injury (AKI).
  • To identify specific miRNAs that show promise for research in human AKI.
  • To highlight methodological limitations in current miRNA research concerning human AKI.

Main Methods:

  • A systematic review and meta-analysis approach was employed.
  • A comprehensive literature search was conducted across multiple databases (Ovid MEDLINE All, Embase, Web of Science, CENTRAL).
  • Studies quantifying miRNA levels in human samples (blood, urine, kidney biopsy) and measuring renal function were included; study quality and heterogeneity were assessed.

Main Results:

  • The review will characterize promising miRNAs for human AKI research.
  • Methodological constraints in current miRNA research for human AKI will be identified.
  • The potential for meta-analysis will be assessed based on study heterogeneity.

Conclusions:

  • There is a lack of randomized trials investigating miRNAs for human AKI prevention or treatment.
  • The quality of included studies and sources of heterogeneity pose challenges to the strength and reproducibility of findings.
  • This review aims to inform future research design by identifying key miRNAs and methodological constraints in human AKI miRNA studies.
Keywords:
acute kidney injurybiomarkerhumanmicroRNAsystematic review

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