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Updated: Nov 5, 2025

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Open-top axially swept light-sheet microscopy.

Bumju Kim1, Myeongsu Na2, Soohyun Park3

  • 1Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea.

Biomedical Optics Express
|May 17, 2021
PubMed
Summary
This summary is machine-generated.

Open-top axially swept light-sheet microscopy (OTAS-LSM) enhances cellular imaging resolution in large tissues. This new technique achieves single-cell resolution for applications like neuronal network imaging.

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Area of Science:

  • Biomedical Imaging
  • Microscopy Techniques
  • Optical Engineering

Background:

  • Open-top light-sheet microscopy (OT-LSM) enables high-throughput imaging of large, cleared tissues.
  • Existing OT-LSM systems exhibit limitations in axial resolution due to weakly focused light sheets.

Purpose of the Study:

  • To develop an open-top axially swept light-sheet microscopy (OTAS-LSM) system.
  • To improve axial resolution for high-throughput cellular imaging of large specimens.

Main Methods:

  • Utilized an electro tunable lens (ETL) to sweep a tightly focused light sheet across the field of view.
  • Employed a camera in rolling shutter mode for emission light collection.
  • Incorporated air objective lenses and a liquid prism, analyzing optical aberrations.

Main Results:

  • Achieved image resolutions under 1.6µm in all directions.
  • Demonstrated single-cell resolution imaging of neuronal networks in cleared mouse brain and small intestine.
  • Successfully applied OTAS-LSM to optically cleared large tissue samples.

Conclusions:

  • OTAS-LSM offers improved axial resolution for high-throughput cellular imaging.
  • The technique is suitable for examining optically cleared large tissues at single-cell resolution.
  • OTAS-LSM presents a valuable tool for advanced biomedical research and diagnostics.