Related Experiment Video
Updated: Jul 25, 2026

11:24
Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
Published on: July 3, 2015
[Fluorescence characteristics of dynamic changes in the DNA structure of peripheral blood cells in irradiated rats]
Abstract:
A simple approach is proposed to determine locally denatured sites and stability characteristics of secondary DNA structure. The method is based on the analysis of the initial part of melting curve and the determination of changes in the optical density of DNA after heating up to a fixed temperature. The potentiality of the approach is illustrated by the experiments with DNA containing defects in the secondary structure caused by gamma-irradiation in vitro. The sensitivity of the method is less than 0.2 Gy.

