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Updated: Nov 5, 2025
Modeling Colitis-Associated Cancer with Azoxymethane AOM and Dextran Sulfate Sodium DSS
Published on: September 11, 2012
Alisol B 23-Acetate Ameliorates Azoxymethane/Dextran Sodium Sulfate-Induced Male Murine Colitis-Associated Colorectal
Huai-Chang Zhu1,2, Xiao-Kang Jia1,2,3, Yong Fan1,2
1College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
Abstract:
Hunting for natural compounds that can modulate the structure of the intestinal flora is a new hotspot for colitis-associated cancer (CAC) prevention or treatment. Alisol B 23-acetate (AB23A) is a natural tetracyclic triterpenoid found in Alismatis rhizoma which is well known for dietary herb. Alismatis rhizoma is often used clinically to treat gastrointestinal diseases in China. In this study, we investigated the potential prevention of AB23A in male mouse models of azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC. AB23A intervention alleviated the body weight loss, disease activity index, colon tumor load, tissue injury, and inflammatory cytokine changes in CAC mice. AB23A intervention leads to remarkable reductions in the activation of TLR, NF-κB and MAPK. AB23A significantly decreased the phosphorylation of p38, ERK, and JNK and up-regulated mucin-2 and the expression of tight junction proteins. The gut microbiota of AB23A-interfered mice was characterized with high microbial diversity, the reduced expansion of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia, and the increased growth of bacteria including Bacteroides, Lactobacillus, and Alloprevotella. These data reveal that AB23A has the potential to be used to treat CAC in the future.
Insights
Alisol B 23-acetate (AB23A), a natural compound, shows promise in preventing colitis-associated cancer (CAC). It alleviates disease symptoms and modulates gut microbiota, offering potential therapeutic benefits for CAC.
Area of Science:
- Gastroenterology and Oncology
- Natural Product Chemistry
- Microbiome Research
Background:
- Colitis-associated cancer (CAC) prevention and treatment are actively researched, with a focus on natural compounds modulating intestinal flora.
- Alisol B 23-acetate (AB23A), a triterpenoid from Alismatis rhizoma, is traditionally used for gastrointestinal ailments.
Purpose of the Study:
- To investigate the potential of AB23A in preventing azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced CAC in male mice.
- To elucidate the molecular mechanisms and gut microbiota changes associated with AB23A intervention in CAC models.
Main Methods:
- Induction of CAC in male mice using AOM and DSS.
- Administration of AB23A and assessment of disease activity, colon tumor load, and tissue injury.
- Analysis of inflammatory cytokine profiles, signaling pathways (TLR, NF-κB, MAPK), and gut microbiota composition.
Main Results:
- AB23A intervention significantly alleviated CAC symptoms, including reduced body weight loss, disease activity index, and colon tumor burden.
- AB23A decreased inflammatory cytokine levels and inhibited the activation of TLR, NF-κB, and MAPK signaling pathways.
- AB23A treatment promoted gut barrier integrity by up-regulating mucin-2 and tight junction proteins, and favorably altered gut microbiota diversity.
Conclusions:
- AB23A demonstrates significant potential for the prevention and treatment of colitis-associated cancer.
- The therapeutic effects of AB23A are mediated through the modulation of inflammatory pathways and the gut microbiome.
- AB23A represents a promising natural compound for future therapeutic strategies against CAC.
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