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Application of MALDI-TOF MS Profiling Coupled With Functionalized Magnetic Enrichment for Rapid Identification of

Jichong Ying1, Wenjing Gao2, Dichao Huang1

  • 1Ningbo No. 6 Hospital, Ningbo, China.

Frontiers in Chemistry
|May 17, 2021
PubMed

Insights

Rapidly identifying pathogens in orthopedic trauma is crucial. A new method using Fc-MBL@Fe3O4 enrichment and MALDI-TOF MS successfully identified Vibrio alginolyticus in a patient with an open leg fracture, showing potential for direct clinical sample analysis.

Area of Science:

  • Microbiology
  • Biotechnology
  • Clinical Diagnostics

Background:

  • Posttraumatic infections pose significant risks in orthopedic trauma, particularly with open fractures.
  • Accurate and rapid pathogen identification is essential for effective clinical diagnosis and antimicrobial therapy.
  • Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a standard for identifying cultured pathogens, but direct analysis of liquid samples requires pre-purification.

Purpose of the Study:

  • To investigate the feasibility of combining Fc-MBL@Fe3O4 enrichment with MALDI-TOF MS for pathogen identification in liquid-cultured clinical samples.
  • To evaluate this novel approach for direct microbial profiling in orthopedic trauma infections.

Main Methods:

  • Utilized Fc-MBL@Fe3O4 nanoparticles for the enrichment of pathogens from briefly pre-cultured liquid media.
  • Employed MALDI-TOF MS for the identification of enriched microbial targets.
  • Applied the combined method to a clinical sample from a patient with an open-leg fracture.

Main Results:

  • Successfully identified pathogens in a liquid-cultured sample from a patient with an open-leg fracture.
  • The method enabled the identification of the opportunistic pathogen *Vibrio alginolyticus*, an uncommon cause of orthopedic trauma infection.
  • Demonstrated the efficacy of Fc-MBL@Fe3O4 enrichment coupled with MALDI-TOF MS for microbial detection.

Conclusions:

  • The combination of Fc-MBL@Fe3O4 enrichment and MALDI-TOF MS profiling shows significant potential for the direct identification of microbes in clinical samples.
  • This approach offers a promising advancement for diagnosing infections in orthopedic trauma patients, especially those involving challenging liquid specimens.
Keywords:
MALDI-TOF MSVibrio alginolyticusmagnetic enrichmentorthopedic trauma infectionpathogen identification

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