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Updated: Aug 18, 2026

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
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Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

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[Relationship between antibody formation in cultures of lymphoid cells and incubation temperature]

M G Agadzhanian, A E Gurvich

    Biulleten' Eksperimental'Noi Biologii I Meditsiny
    |December 1, 1977
    PubMed

    An increase of the plaque-forming cells (PFC) and of the 3H-thymidine incorporation in the spleen cell cultures of immunized and nonimmunized C57BL/6 mice were studied in increase of the incubation temperature from 2 to 37 degrees C. An exponential rise of the PFC cells with the elevation of temperature and the presence of the "breaking" temperature, above which the rate of increase of the PFC cells displayed a sharp elevation, was demonstrated. The curves of intensification of the 3H-thymidine incorporation with the temperature elevation failed to follow the pattern of the PFC growth curve in a number of cases. Cultivation of immune cells at low temperatures led to the accumulation in the medium of some factors simulating the AFC formation.

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