A long-term retaining molecular coating for corneal regeneration

Area of Science:

• Biomaterials Science
• Ophthalmology
• Regenerative Medicine

Background:

• Corneal injuries cause surface diseases and potential blindness globally.
• A need exists for practical biomaterials to enhance corneal regeneration.
• Current treatments face challenges in efficacy and feasibility.

Purpose of the Study:

• To develop and evaluate a novel protein coating for accelerating corneal regeneration.
• To investigate the potential of modified gelatin as a corneal repair agent.
• To assess the feasibility of an eye drop formulation for corneal injury treatment.

Main Methods:

• Synthesis of o-nitrosobenzaldehyde group (NB)-modified gelatin (GelNB).
• Application of GelNB as a thin molecular coating on corneal surfaces via covalent bonding.
• Evaluation of corneal regeneration, cell migration, and histological structure post-treatment.

Main Results:

• GelNB coating adhered directly to the corneal surface, forming a stable layer.
• The coating effectively accelerated corneal repair and regeneration.
• Regenerated corneal tissue exhibited histological structures similar to the native cornea.
• The molecular coating provided a favorable environment for cell migration and avoided rapid clearance.

Conclusions:

• o-nitrosobenzaldehyde group (NB)-modified gelatin (GelNB) is a promising biomaterial for corneal regeneration.
• The molecular coating strategy offers a convenient eye drop solution for corneal injuries.
• This approach presents a feasible method to combat corneal diseases and prevent blindness.